Effects of platelet-rich plasma gel on skin healing in surgical wound in horses

• Autor(es):

  DeRossi, Rafael (UFMS. Faculty of Veterinary Medicine and Animal Science. Department of Veterinary Medicine, Surgery and Anesthesiology);

  Coelho, Anna Carolina Anciliero de Oliveira;

  Mello, Gisele Silveira de;

  Frazílio, Fabrício Oliveira (University for Development of the State and the Pantanal Region. Division of Surgery);

  Leal, Cássia Rejane Brito (UFMS. Post-graduate in Health and Development, West Central Region);

  Facco, Gilberto Gonçalves (UNIDERP. Division of Pathology);

  Brum, Karine Bonucielli (UFMS. Department of Pathology).

• Fonte:

  Acta Cirurgica Brasileira; volume 24, número 4, páginas 276-281. Agosto 2009.

• Assuntos:

  Platelet-Rich Plasma;

  Wound Healing;

  Skin;

  Horses.

• Resumo:

  PURPOSE: To establish a low-cost method to prepare platelet-rich plasma (PRP) and evaluates the potential of platelet derived factors to enhance wound healing in the surgical wounds in equine. METHODS: To obtain a PRP gel, calcium gluconate and autologous thrombin were added to platelet-rich plasma. For the tests six saddle horses were used and two surgical incisions were made in each animal. Wounds were treated with PRP gel or untreated. Sequential wound biopsies collected at Treatment 1: at days 5 and 30 and Treatment 2: at days 15 and 45 post wounding permitted comparison of differentiation markers and wound repair. RESULTS: The optimal platelets enrichment over 4.0 time's baseline values was obtained using 300 g for 10 min on the first centrifugation and 640 g for 10 min on the second centrifugation. CONCLUSION: Wounds treated with PRP gel exhibit more rapid epithelial differentiation and enhanced organization of dermal collagen compared to controls in equine

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