Apoptotic effects of inositol hexaphosphate on biomarker Itpr3 in induced colon rat carcinogenesis

• Autor(es):

  Guido, Marks (UNIFESP-SP. Department of Surgery. Program in Surgery and Experimentation);

  Fagundes, Djalma José (UNIFESP-SP. Department of Surgery. Division of Operative Technique and Experimental Surgery);

  Ynouye, Celso Massaschi (UFMS-MS. Department of Surgery);

  Pontes, Elenir Rose Jardim Cury (UFMS-MS. Department of Food Technology and Public Health);

  Takita, Luiz Carlos (UFMS-MS. Department of Surgery);

  Amaral, Eva Glória Siufi do (UFMS-MS. Department of Surgery);

  Teruya, Roberto (UFMS-MS. Department of Surgery);

  Paes, Manoel Catarino (UFMS-MS. Department of Surgery);

  Brasileiro, José Lacerda (UFMS-MS. Department of Surgery);

  Aydos, Ricardo Dutra (UFMS-MS. Department of Surgery).

• Fonte:

  Acta Cirurgica Brasileira; volume 23, número 2, páginas 157-164. Abril 2008.

• Assuntos:

  Apoptosis;

  Azoxymethane;

  Cancer;

  Colon;

  Inositol 1,4,5 trisphosphate receptors;

  Computer-assisted image processing;

  Phytic acid;

  Rats.

• Resumo:

  PURPOSE: To study the effect of the modulation of inositol hexaphosphate (IP6) in the biological immunohistochemistry expression of cellular signaling marker apoptosis, in model of carcinogenesis of colon induced by azoxymethane (AOM). METHODS: Wistar rats (N=112) distributed in 4 groups (n=28): Control; B, AOM (5 mg kg-1, 2x, to break week 3); C, IP6 (in water 1%, six weeks); D, IP6+AOM. Weekly euthanasia (n=7), from week three. Immunohistochemistry of ascendant colon with biological marker inositol 1,4,5 triphosphate receptor type III (Itpr3). Quantification of the immune-expression with use of computer-assisted image processing. Analysis statistics of the means between groups, weeks in groups, groups in weeks, and established significance when p<0.05. RESULTS: One proved significant difference between groups in the expression of Itpr3, p<0.0001; with Itpr3 reduction of BxD group, p<0.001. CONCLUSION: Inositol hexaphosphate promotes modulation of biological markers with reduction of Itpr3 in carcinogenesis of colon.

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