Evaluation of an experimental model of necrotizing enterocolitis in rats

• Autor(es):

  Meyer, Karine Furtado (UNIFESP. EPM);

  Martins, José Luiz (UNIFESP. EPM. Departamento de Cirurgia);

  Freitas Filho, Luiz Gonzaga de (UNIFESP. EPM. Departamento de Cirurgia);

  Oliva, Maria Luiza Vilela (UNIFESP. EPM. Departamento de Bioquímica);

  Patrício, Francy Reis da Silva (UNIFESP. EPM. Departamento de Patologia);

  Macedo, Maurício (UNIFESP. EPM);

  Wang, Lina (Hospital do Servidor Público Estadual de São Paulo).

• Fonte:

  Acta Cirurgica Brasileira; volume 21, número 2, páginas 113-118. Abril 2006.

• Assuntos:

  Enterocolitis, Necrotizing;

  Models, Animal;

  Rats.

• Resumo:

  OBJECTIVE: To evaluate an experimental model of necrotizing enterocolitis in rats proposed by OKUR e col. in 1995. METHODS: On their first day of life, 28 EPM-Wistar rats weighing between 4 and 6 grams were submitted to hypoxia (H) by placing them in a CO2 gas chamber for rodents' sacrifice, where they received a 100% CO2 air flow for 5 minutes. After the hypoxia the animals were reanimated (R) with a 100% O2 air flow, also for 5 minutes. The animals were allocated in two groups: G1: control (n=12): rats not submitted to H-R; G2: (n=16): rats submitted to H-R. Segments of the small intestine and colon were prepared for histological analysis. The remaining intestine was used to measure tissular malondialdehyde. RESULTS: Mean malondialdehyde dosages were 1.05 (0.44-2.03) and 2.60 (0.59- 6.4) nmol MDA/mg protein for G1 and G2, respectively. G2's mean value was significantly higher than in the control group (p<0.002). Significant statistical difference between the studied groups was found in relation the level of injury, with G1 presenting significantly lower levels than G2. CONCLUSIONS: The model showed that neonatal hypoxia may cause intestinal wall injury in rats. Despite the discreet histological injuries found, the method is suitable for evaluation of tissular free radicals.

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